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Description

Petroleum refineries around the world have generated solid wastes during refining process and stocking of crude oil. This leads to critical effects in the environment. Thus remediation of these hydrocarbons by natural decontamination is of at most importance. Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons. In this study I have investigated the ability of microorganism present in the soil sample collected from petroleum contaminated area of Kalady, Kerala, to degrade the hydrocarbons. The soil samples collected were initially screened for diesel degraders by culturing isolates in BH media with diesel as carbon and energy source . The diesel oil degraders were identified as bacillus and pseudomonas based on the morphological and biochemical studies The ability of these microorganisms to grow by using diesel as carbon source were analysed by culturing them in BHMS broth and evaluating their growth colourimetrically